polyvinylidene fluoride scaffolds immobilized with a vitronectin-derived peptide

Use of Alcalase in the production of bioactive peptides: A review 

This review aims to cover the uses of the commercially available protease Alcalase in the production of biologically active peptides since 2010. Immobilization of Alcalase has also been reviewed, as immobilization of the enzyme may improve the final reaction design enabling the use of more drastic conditions and the reuse of the biocatalyst.

That way, this review presents the production, via Alcalase hydrolysis of different proteins, of peptides with antioxidant, angiotensin I-converting enzyme inhibitory, metal binding, antidiabetic, anti-inflammatory and antimicrobial activities (among other bioactivities) and peptides that improve the functional, sensory and nutritional properties of foods.

Alcalase has proved to be among the most efficient proteases for this goal, using different protein sources, being especially interesting the use of the protein residues from food industry as feedstock, as this also solves nature pollution problems.

Very interestingly, the bioactivities of the protein hydrolysates further improved when Alcalase is used in a combined way with other proteases both in a sequential way or in a simultaneous hydrolysis (something that could be related to the concept of combi-enzymes), as the combination of proteases with different selectivities and specificities enable the production of a larger amount of peptides and of a smaller size.

Development of A2G80 peptide-gene complex for targeted delivery to muscle cells

 Therapeutic strategies based on antisense oligonucleotides and therapeutic genes are being extensively investigated for the treatment of hereditary muscle diseases and hold great promise. However, the cellular uptake of these polyanions to the muscle cells is inefficient. Therefore, it is necessary to develop more effective methods of gene delivery into the muscle tissue.

• The A2G80 peptide (VQLRNGFPYFSY) from the laminin α2 chain has high affinity for α-dystroglycan (α-DG) which is expressed on the membrane of muscle cells. In this study, we designed a peptide-modified A2G80 with oligoarginine and oligohistidine (A2G80-R9-H8), and prepared peptide/plasmid DNA (pDNA) complex, to develop an efficient gene delivery system for the muscle tissue.

• The peptide/pDNA complex showed α-DG-dependent cellular uptake of the A2G80 sequence and significantly improved gene transfection efficiency mediated by the oligohistidine sequence in C2C12 myoblast cells. Further, the peptide/pDNA complex promoted efficient and sustained gene expression in the Duchenne muscular dystrophy mouse models. The A2G80-R9-H8 peptide has the potential for use as a specific carrier for targeting muscle in gene therapy in muscular dystrophy.

Synthetic peptide hydrogels as 3D scaffolds for tissue engineering

 The regeneration of tissues and organs poses an immense challenge due to the extreme complexity in the research work involved. Despite the tissue engineering approach being considered as a promising strategy for more than two decades, a key issue impeding its progress is the lack of ideal scaffold materials.

Nature-inspired synthetic peptide hydrogels are inherently biocompatible, and its high resemblance to extracellular matrix makes peptide hydrogels suitable 3D scaffold materials. This review covers the important aspects of peptide hydrogels as 3D scaffolds, including mechanical properties, biodegradability and bioactivity, and the current approaches in creating matrices with optimized features. Many of these scaffolds contain peptide sequences that are widely reported for tissue repair and regeneration and these peptide sequences will also be discussed.

Furthermore, 3D biofabrication strategies of synthetic peptide hydrogels and the recent advances of peptide hydrogels in tissue engineering will also be described to reflect the current trend in the field. In the final section, we will present the future outlook in the design and development of peptide-based hydrogels for translational tissue engineering applications.

Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes 

• Most linear peptides directly interact with membranes, but the mechanisms of interaction are far from being completely understood. Here, we present an investigation of the membrane interactions of a designed peptide containing a non-natural, synthetic amino acid. We selected a nonapeptide that is reported to interact with phospholipid membranes, ALYLAIRKR, abbreviated as ALY.

• We designed a modified peptide (azoALY) by substituting the tyrosine residue of ALY with an antimicrobial azobenzene-bearing amino acid. Both of the peptides were examined for their ability to interact with model membranes, assessing the penetration of phospholipid monolayers, and leakage across the bilayer of large unilamellar vesicles (LUVs) and giant unilamellar vesicles (GUVs).

• The latter was performed in a microfluidic device in order to study the kinetics of leakage of entrapped calcein from the vesicles at the single vesicle level. Both types of vesicles were prepared from a 9:1 (mol/mol) mixture of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(1′-rac-glycerol). Calcein leakage from the vesicles was more pronounced at a low concentration in the case of azoALY than for ALY.

• Increased vesicle membrane disturbance in the presence of azoALY was also evident from an enzymatic assay with LUVs and entrapped horseradish peroxidase. Molecular dynamics simulations of ALY and azoALY in an anionic POPC/POPG model bilayer showed that ALY peptide only interacts with the lipid head groups.
• In contrast, azoALY penetrates the hydrophobic core of the bilayers causing a stronger membrane perturbation as compared to ALY, in qualitative agreement with the experimental results from the leakage assays.

Glypican-6 Antibody (FITC)
20-abx106888	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Glypican 6 (GPC6) Antibody
20-abx005979	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Glypican 6 (GPC6) Antibody
20-abx211678	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Glypican 6 (GPC6) Antibody
20-abx212986	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Glypican 6 (GPC6) Antibody
20-abx213390	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Human glypican 6 (GPC6) ELISA kit
CSB-EL009708HU-24T	Cusabio	1 plate of 24 wells	EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human glypican 6 (GPC6) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human glypican 6 (GPC6) ELISA kit
1-CSB-EL009708HU	Cusabio	EUR 964.80 EUR 6118.80 EUR 3244.80	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human glypican 6 (GPC6) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Glypican 6(GPC6)ELISA Kit
QY-E02274	Qayee Biotechnology	96T	EUR 433.2

Recombinant Human Glypican-6 Protein, His, Yeast-100ug
QP9160-ye-100ug	EnQuireBio	100ug	EUR 576

Recombinant Human Glypican-6 Protein, His, Yeast-10ug
QP9160-ye-10ug	EnQuireBio	10ug	EUR 283.2

Recombinant Human Glypican-6 Protein, His, Yeast-1mg
QP9160-ye-1mg	EnQuireBio	1mg	EUR 2262

Recombinant Human Glypican-6 Protein, His, Yeast-200ug
QP9160-ye-200ug	EnQuireBio	200ug	EUR 892.8

Recombinant Human Glypican-6 Protein, His, Yeast-500ug
QP9160-ye-500ug	EnQuireBio	500ug	EUR 1447.2

Recombinant Human Glypican-6 Protein, His, Yeast-50ug
QP9160-ye-50ug	EnQuireBio	50ug	EUR 358.8

Mouse Glypican-6,GPC-6 ELISA Kit
YLA1311MO-48T	Shanghai YL Biotech	48T	EUR 465

Mouse Glypican-6,GPC-6 ELISA Kit
YLA1311MO-96T	Shanghai YL Biotech	96T	EUR 600

Individual Reaction Mix 6
G065-6	ABM	200 reactions	EUR 200.4

Random Nanofibers 6 Well Plate
3D00006-6	Neuromics	700 nm-PCLs	EUR 111.6

Aligned Nanofibers 6 Well Plate
3D00012-6	Neuromics	700 nm-PCLs	EUR 116.4

pLenti-CLDN1 shRNA-6 Plasmid
PVTBAV04867-6	Lifescience Market	2 ug	EUR 427.2

Tissue Culture Plate, 6 Well
TCP20-6	Bio Basic	1 UNIT	EUR 64.18

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 6 vials)
M1040-6	Biovision	each	EUR 1560

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 6 vials)
M1041-6	Biovision	each	EUR 1886.4

ExoStd? Lyophilized Exosome Standard (30 µg, Human Serum, 6 vials)
M1042-6	Biovision	each	EUR 1560

ExoStd? Lyophilized Exosome Standard (100 µg, Human Serum, 6 vials)
M1043-6	Biovision	each	EUR 1886.4

ExoStd? Lyophilized Exosome Standard (30 µg, Human Urine, 6 vials)
M1044-6	Biovision	each	EUR 1546.8

ExoStd? Lyophilized Exosome Standard (100 µg, Human Urine, 6 vials)
M1045-6	Biovision	each	EUR 1886.4

ExoStd? Lyophilized Exosome Standard (30 µg, Human Saliva, 6 vials)
M1046-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, Human Saliva, 6 vials)
M1047-6	Biovision	each	EUR 1945.2

Aligned Nanofibers 6 Well Plate Inserts
3D00016-6	Neuromics	700 nm-PCLs	EUR 117.6

Pesticide Mix 6 Containing 15 Compounds in Acetonitrile
SPXPR-6	Scientific Laboratory Supplies	1ML	EUR 182.4

ExoStd? Lyophilized Exosome Standard (30 µg, U87 MG, 6 vials)
M1054-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, U87 MG, 6 vials)
M1055-6	Biovision	each	EUR 1939.2

Human GPC6 (Glypican- 6) ELISA Kit (CUSTOM ELISA KIT)
ELI-48039h	Lifescience Market	96 Tests	EUR 988.8

ExoStd? Lyophilized Exosome Standard (30 µg, COLO1 cell line, 6 vials)
M1048-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, COLO1 cell line, 6 vials)
M1049-6	Biovision	each	EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, MM1 cell line, 6 vials)
M1050-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, MM1 cell line, 6 vials)
M1051-6	Biovision	each	EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, BLCL21 cell line, 6 vials)
M1052-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, BLCL21 cell line, 6 vials)
M1053-6	Biovision	each	EUR 1886.4

ExoStd? Lyophilized Exosome Standard (30 µg, HCT116 cell line, 6 vials)
M1058-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, HCT116 cell line, 6 vials)
M1059-6	Biovision	each	EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, PC3 cell line, 6 vials)
M1060-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, PC3 cell line, 6 vials)
M1061-6	Biovision	each	EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, DAUD1 cell line, 6 vials)
M1064-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, DAUD1 cell line, 6 vials)
M1065-6	Biovision	each	EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, A549 cell line, 6 vials)
M1066-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, A549 cell line, 6 vials)
M1067-6	Biovision	each	EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, B16F10 cell line, 6 vials)
M1070-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (1090 µg, B16F10 cell line, 6 vials)
M1071-6	Biovision	each	EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, BPH-1 cell line, 6 vials)
M1062-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, BPH-1 cell line, 6 vials)
M1063-6	Biovision	each	EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, K-562 cell line, 6 vials)
M1068-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, K-562 cell line, 6 vials)
M1069-6	Biovision	each	EUR 1939.2

ExoStd? Lyophilized Exosome Standard (30 µg, SK-N-SH cell line, 6 vials)
M1056-6	Biovision	each	EUR 1567.2

ExoStd? Lyophilized Exosome Standard (100 µg, SK-N-SH cell line, 6 vials)
M1057-6	Biovision	each	EUR 1939.2

Human Glypican 1 (GPC1) Protein
20-abx066889	Abbexa	EUR 627.60 EUR 292.80 EUR 1796.40 EUR 727.20 EUR 460.80	100 ug 10 ug 1 mg 200 ug 50 ug

Human Glypican 3 (GPC3) Protein
20-abx066892	Abbexa	EUR 744.00 EUR 326.40 EUR 2230.80 EUR 878.40 EUR 543.60	100 ug 10 ug 1 mg 200 ug 50 ug

Human Glypican 4 (GPC4) Protein
20-abx066893	Abbexa	EUR 895.20 EUR 360.00 EUR 2798.40 EUR 1062.00 EUR 627.60	100 ug 10 ug 1 mg 200 ug 50 ug

Human Glypican 3 (GPC3) Protein
abx060155-100ug	Abbexa	100 ug	EUR 1362

Human Glypican 3 (GPC3) Protein
abx060263-1mg	Abbexa	1 mg	EUR 2532

Human Glypican-1 (GPC1) Protein
abx060282-100ug	Abbexa	100 ug	EUR 944.4

Human Glypican 2 (GPC2) Protein
20-abx653616	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

Recombinant Human Glypican 3 Protein
RP00138	Abclonal	10 μg	EUR 186

Recombinant Human Glypican 1 Protein
RP00297	Abclonal	20 μg	EUR 231.6

Recombinant Human HGF Protein
PROTP14210-6	BosterBio	10ug	EUR 380.4
Description: HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues.*Manufactured using (BTI-Tn-5B1-4) cells under license from Boyce Thompson Institute for Plant Research, Inc.

Recombinant Human Myostatin Protein
PROTO14793-6	BosterBio	10ug	EUR 380.4
Description: Myostatin is a TGF-β family member that acts as an inhibitor of skeletal muscle growth. This muscle-specific cytokine interacts with Activin type I and type II receptors, and suppresses myoblast proliferation by arresting cell-cycle in the G1 phase. Suppression of myostatin activity facilitates muscle formation and may be useful in reducing and/or preventing adiposity and type-2 diabetes. Myostatin activity can be blocked by the Activin-binding protein Follistatin, and by the propeptide of Myostatin. Recombinant Human myostatin is a 25.0 kDa protein consisting of two identical 109 amino acid polypeptides linked by a single disulfide bond.

Acrp30 Human, Adiponectin Human Recombinant Protein, HEK
PROTQ15848-6	BosterBio	Regular: 10ug	EUR 380.4
Description: The Acrp30 Human Recombinant produced in HEK293 cells is fused with FLAG tag.

Canadian Pesticide Mix 6 Containing 11 Compounds
CAN-CAN-6	Scientific Laboratory Supplies	1ML	EUR 84.36

Recombinant Human IL-7 Protein
PROTP13232-6	BosterBio	10ug	EUR 380.4
Description: IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant human IL-7 is a 17.4 kDa protein containing 153 amino acid residues.

Recombinant Human PlGF-3 Protein
PROTP49763-6	BosterBio	25ug	EUR 380.4
Description: PlGF-3 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PlGF-3 is expressed exclusively in the placenta. It signals through the VEGFR-1/FLT1 receptor and stimulates endothelial cell proliferation and migration. PlGF-3 lacks heparin binding affinity. Recombinant human PlGF-3 is a 45.7 kDa disulfide-linked homodimeric protein of two 203 amino acid polypeptide chains.

Recombinant Human GM-CSF Protein
PROTP04141-6	BosterBio	20ug	EUR 380.4
Description: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.

BCL2 Human, B-Cell Lymphoma Protein 2 Alpha Human Recombinant Protein, His Tag
PROTP10415-6	BosterBio	Regular: 20ug	EUR 380.4
Description: BCL2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 1-211 and having a molecular mass of 25.4 kDa. The BCL2 is fused to a 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Human IL-1 beta Recombinant Protein
R00101-6	BosterBio	5ug/vial	EUR 310.8
Description: IL-1 beta (IL-1β) is a member of the interleukin 1 family of cytokines. The IL-1 beta cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. Human IL-1 beta Recombinant Protein is purified interleukin-1 beta cytokine produced in yeast.

22 gauge small hub needles (Pack of 6)
MLCP-HN22-6	MiTeGen	6 X 22 GUAGE	EUR 54
Description: 22 gauge small hub needles (Pack of 6)

VEGF Human, Vascular Endothelial Growth Factor Human Recombinant Protein, His
PROTP15692-6	BosterBio	Regular: 20ug	EUR 380.4
Description: Vascular Endothelial Growth Factor Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain (aa 207-371) containing a total of 185 amino acids and having a molecular mass of 21.3 kDa (corresponding to Isoform L-VEGF165 UniProt acc#P15692-11). ;The VEGF is fused to a 20 a.a His-tag at N-terminus and is purified by proprietary chromatographic techniques.

Recombinant Human FGF-basic (154 a.a.) Protein
PROTP09038-6	BosterBio	50ug	EUR 380.4
Description: FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.

Recombinant Human Oncostatin M (227 a.a.) Protein
PROTP13725-6	BosterBio	10ug	EUR 380.4
Description: Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi's sarcoma cells, OSH can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi's sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells. OSM share several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells. Recombinant human Oncostatin M is a 25.7 kDa protein, containing 227 amino acid residues.

Recombinant Human SDF-1 Beta (CXCL12) Protein
PROTP48061-6	BosterBio	10ug	EUR 380.4
Description: SDF-1α and β are stromal derived CXC chemokines, and signal through the CXCR4 receptor. SDF-1α and β chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV suppressive activity in cells expressing the CXCR4 receptor. Recombinant human SDF-1β is an 8.5 kDa protein containing 72 amino acid residues.

Glypican 1 Recombinant Protein
91-578	ProSci	0.05 mg	EUR 588.3
Description: Glypican-1 is a cell membrane protein and belongs to the glypican family. The protein may act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. It is required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.

Glypican 4 (GPC4) Protein
20-abx262759	Abbexa	EUR 393.60 EUR 7676.40 EUR 276.00	10 ug 1 mg 2 µg

Glypican 3 Recombinant Protein
96-360	ProSci	0.1 mg	EUR 682.8
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein
96-803	ProSci	0.1 mg	EUR 852.9
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein
96-804	ProSci	0.1 mg	EUR 651.3
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein
97-001	ProSci	0.1 mg	EUR 682.8
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein
97-002	ProSci	0.1 mg	EUR 651.3
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

GPC3 Glypican-3 Human Recombinant Protein
PROTP51654	BosterBio	Regular: 10ug	EUR 380.4
Description: GPC3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 544 amino acids (25-559a.a.) and having a molecular mass of 61.8kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). GPC3 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.

GPC4 Glypican-4 Human Recombinant Protein
PROTO75487	BosterBio	Regular: 10ug	EUR 380.4
Description: GPC4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids (401-529a.a) and having a molecular mass of 18kDa.GPC4 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Mouse GPC6 (Glypican- 6) ELISA Kit (CUSTOM ELISA KIT)
ELI-27268m	Lifescience Market	96 Tests	EUR 1038

IC Multi Elem (6) Anion Std 10ppm in H2O
ICA-10PPM-6	Scientific Laboratory Supplies	100ML	EUR 269.04

Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin-derived peptide

Polyvinylidene fluoride (PVDF) is biocompatible, easy to fabricate, and has piezoelectric properties; it has been used for many biomedical applications including stem cell engineering. However, long-term cultivation of human embryonic stem cells (hESCs) and their differentiation toward cardiac lineages on PVDF have not been investigated.

Herein, PVDF nanoscaled membrane scaffolds were fabricated by electrospinning; a vitronectin-derived peptide-mussel adhesive protein fusion (VNm) was immobilized on the scaffolds. hESCs cultured on the VNm-coated PVDF scaffold (VNm-PVDF scaffold) were stably expanded for more than 10 passages while maintaining the expression of pluripotency markers and genomic integrity.

Under cardiac differentiation conditions, hESCs on the VNm-PVDF scaffold generated more spontaneously beating colonies and showed the upregulation of cardiac-related genes, compared with those cultured on Matrigel and VNm alone. Thus, VNm-PVDF scaffolds may be suitable for the long-term culture of hESCs and their differentiation into cardiac cells, thus expanding their application in regenerative medicine.