polyvinylidene fluoride scaffolds immobilized with a vitronectin-derived peptide

Use of Alcalase in the production of bioactive peptides: A review 

This review aims to cover the uses of the commercially available protease Alcalase in the production of biologically active peptides since 2010. Immobilization of Alcalase has also been reviewed, as immobilization of the enzyme may improve the final reaction design enabling the use of more drastic conditions and the reuse of the biocatalyst.

That way, this review presents the production, via Alcalase hydrolysis of different proteins, of peptides with antioxidant, angiotensin I-converting enzyme inhibitory, metal binding, antidiabetic, anti-inflammatory and antimicrobial activities (among other bioactivities) and peptides that improve the functional, sensory and nutritional properties of foods.

Alcalase has proved to be among the most efficient proteases for this goal, using different protein sources, being especially interesting the use of the protein residues from food industry as feedstock, as this also solves nature pollution problems.

Very interestingly, the bioactivities of the protein hydrolysates further improved when Alcalase is used in a combined way with other proteases both in a sequential way or in a simultaneous hydrolysis (something that could be related to the concept of combi-enzymes), as the combination of proteases with different selectivities and specificities enable the production of a larger amount of peptides and of a smaller size.

Development of A2G80 peptide-gene complex for targeted delivery to muscle cells

 Therapeutic strategies based on antisense oligonucleotides and therapeutic genes are being extensively investigated for the treatment of hereditary muscle diseases and hold great promise. However, the cellular uptake of these polyanions to the muscle cells is inefficient. Therefore, it is necessary to develop more effective methods of gene delivery into the muscle tissue.

• The A2G80 peptide (VQLRNGFPYFSY) from the laminin α2 chain has high affinity for α-dystroglycan (α-DG) which is expressed on the membrane of muscle cells. In this study, we designed a peptide-modified A2G80 with oligoarginine and oligohistidine (A2G80-R9-H8), and prepared peptide/plasmid DNA (pDNA) complex, to develop an efficient gene delivery system for the muscle tissue.

• The peptide/pDNA complex showed α-DG-dependent cellular uptake of the A2G80 sequence and significantly improved gene transfection efficiency mediated by the oligohistidine sequence in C2C12 myoblast cells. Further, the peptide/pDNA complex promoted efficient and sustained gene expression in the Duchenne muscular dystrophy mouse models. The A2G80-R9-H8 peptide has the potential for use as a specific carrier for targeting muscle in gene therapy in muscular dystrophy.

Synthetic peptide hydrogels as 3D scaffolds for tissue engineering

 The regeneration of tissues and organs poses an immense challenge due to the extreme complexity in the research work involved. Despite the tissue engineering approach being considered as a promising strategy for more than two decades, a key issue impeding its progress is the lack of ideal scaffold materials.

Nature-inspired synthetic peptide hydrogels are inherently biocompatible, and its high resemblance to extracellular matrix makes peptide hydrogels suitable 3D scaffold materials. This review covers the important aspects of peptide hydrogels as 3D scaffolds, including mechanical properties, biodegradability and bioactivity, and the current approaches in creating matrices with optimized features. Many of these scaffolds contain peptide sequences that are widely reported for tissue repair and regeneration and these peptide sequences will also be discussed.

Furthermore, 3D biofabrication strategies of synthetic peptide hydrogels and the recent advances of peptide hydrogels in tissue engineering will also be described to reflect the current trend in the field. In the final section, we will present the future outlook in the design and development of peptide-based hydrogels for translational tissue engineering applications.

Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes 

• Most linear peptides directly interact with membranes, but the mechanisms of interaction are far from being completely understood. Here, we present an investigation of the membrane interactions of a designed peptide containing a non-natural, synthetic amino acid. We selected a nonapeptide that is reported to interact with phospholipid membranes, ALYLAIRKR, abbreviated as ALY.

• We designed a modified peptide (azoALY) by substituting the tyrosine residue of ALY with an antimicrobial azobenzene-bearing amino acid. Both of the peptides were examined for their ability to interact with model membranes, assessing the penetration of phospholipid monolayers, and leakage across the bilayer of large unilamellar vesicles (LUVs) and giant unilamellar vesicles (GUVs).

• The latter was performed in a microfluidic device in order to study the kinetics of leakage of entrapped calcein from the vesicles at the single vesicle level. Both types of vesicles were prepared from a 9:1 (mol/mol) mixture of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(1′-rac-glycerol). Calcein leakage from the vesicles was more pronounced at a low concentration in the case of azoALY than for ALY.

• Increased vesicle membrane disturbance in the presence of azoALY was also evident from an enzymatic assay with LUVs and entrapped horseradish peroxidase. Molecular dynamics simulations of ALY and azoALY in an anionic POPC/POPG model bilayer showed that ALY peptide only interacts with the lipid head groups.
• In contrast, azoALY penetrates the hydrophobic core of the bilayers causing a stronger membrane perturbation as compared to ALY, in qualitative agreement with the experimental results from the leakage assays.

Human Glypican-1 (GPC1) Protein
abx060282-100ug	Abbexa	100 ug	EUR 944.4

Recombinant Human Glypican 3 Protein
RP00138	Abclonal	10 μg	EUR 186

Recombinant Human Glypican 1 Protein
RP00297	Abclonal	20 μg	EUR 231.6

Human Glypican 1 (GPC1) Protein
20-abx066889	Abbexa	EUR 627.60 EUR 292.80 EUR 1796.40 EUR 727.20 EUR 460.80	100 ug 10 ug 1 mg 200 ug 50 ug

Human Glypican 3 (GPC3) Protein
20-abx066892	Abbexa	EUR 744.00 EUR 326.40 EUR 2230.80 EUR 878.40 EUR 543.60	100 ug 10 ug 1 mg 200 ug 50 ug

Human Glypican 4 (GPC4) Protein
20-abx066893	Abbexa	EUR 895.20 EUR 360.00 EUR 2798.40 EUR 1062.00 EUR 627.60	100 ug 10 ug 1 mg 200 ug 50 ug

Human Glypican 3 (GPC3) Protein
abx060155-100ug	Abbexa	100 ug	EUR 1362

Human Glypican 3 (GPC3) Protein
abx060263-1mg	Abbexa	1 mg	EUR 2532

Human Glypican 2 (GPC2) Protein
20-abx653616	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

Human Glypican-6,GPC-6 ELISA Kit
YLA3605HU-48T	Shanghai YL Biotech	48T	EUR 435

Human Glypican-6,GPC-6 ELISA Kit
YLA3605HU-96T	Shanghai YL Biotech	96T	EUR 562.5

Human Glypican 3 Protein (Gln 25-His 559)
VAng-1696Lsx-100g	Creative Biolabs	100 µg	EUR 1116
Description: Human Glypican 3 (GPC3) is expressed in human 293 cells. (Uniprot ID: P51654-1)

Human Glypican 3 Protein (Gln 25-His 559)
VAng-1696Lsx-1mg	Creative Biolabs	1 mg	EUR 5998.8
Description: Human Glypican 3 (GPC3) is expressed in human 293 cells. (Uniprot ID: P51654-1)

Recombinant Human Glypican 3 Protein, His Tag
E40KMP1201	EnoGene	20ug	EUR 495

Recombinant Human Glypican 1 Protein, His Tag
E40KMP1589	EnoGene	20ug	EUR 495

Human Glypican 2 Protein (Ser 24-Gly 554) [Fc]
VAng-1694Lsx-100g	Creative Biolabs	100 µg	EUR 1215.6
Description: Human Glypican 2 (GPC2), Fc Tag, is expressed in human 293 cells. (Uniprot ID: Q8N158-1)

Human Glypican 2 Protein (Ser 24-Gly 554) [Fc]
VAng-1694Lsx-1mg	Creative Biolabs	1 mg	EUR 7682.4
Description: Human Glypican 2 (GPC2), Fc Tag, is expressed in human 293 cells. (Uniprot ID: Q8N158-1)

Human Glypican 2 Protein (Ser 24-Gly 554) [His]
VAng-1695Lsx-100g	Creative Biolabs	100 µg	EUR 1215.6
Description: Human Glypican 2 (GPC2), His Tag, is expressed in human 293 cells. (Uniprot ID: Q8N158-1)

Human Glypican 2 Protein (Ser 24-Gly 554) [His]
VAng-1695Lsx-1mg	Creative Biolabs	1 mg	EUR 7682.4
Description: Human Glypican 2 (GPC2), His Tag, is expressed in human 293 cells. (Uniprot ID: Q8N158-1)

Human Glypican 1 Protein (Asp 24-Ser 530) [Fc]
VAng-1692Lsx-100g	Creative Biolabs	100 µg	EUR 1215.6
Description: Human Glypican 1 (GPC1), Fc Tag, is expressed in human 293 cells. (Uniprot ID: P35052-1)

Human Glypican 1 Protein (Asp 24-Ser 530) [Fc]
VAng-1692Lsx-1mg	Creative Biolabs	1 mg	EUR 7682.4
Description: Human Glypican 1 (GPC1), Fc Tag, is expressed in human 293 cells. (Uniprot ID: P35052-1)

Human Glypican 3 Protein (Gln 25-His 559) [Fc]
VAng-1697Lsx-100g	Creative Biolabs	100 µg	EUR 1050
Description: Human Glypican 3 (GPC3), Fc tag, is expressed in human 293 cells. (Uniprot ID: NP_004475)

Human Glypican 3 Protein (Gln 25-His 559) [Fc]
VAng-1697Lsx-1mg	Creative Biolabs	1 mg	EUR 6592.8
Description: Human Glypican 3 (GPC3), Fc tag, is expressed in human 293 cells. (Uniprot ID: NP_004475)

Human Glypican 1 Protein (Asp 24-Ser 530) [His]
VAng-1693Lsx-100g	Creative Biolabs	100 µg	EUR 1215.6
Description: Human Glypican 1 (GPC1), His Tag, is expressed in human 293 cells. (Uniprot ID: P35052-1)

Human Glypican 1 Protein (Asp 24-Ser 530) [His]
VAng-1693Lsx-1mg	Creative Biolabs	1 mg	EUR 7682.4
Description: Human Glypican 1 (GPC1), His Tag, is expressed in human 293 cells. (Uniprot ID: P35052-1)

Human Glypican 3 Protein (Gln 25-His 559) [His]
VAng-1699Lsx-100g	Creative Biolabs	100 µg	EUR 1116
Description: Human Glypican 3 (GPC3), His tag, is expressed in human 293 cells. (Uniprot ID: P51654)

Human Glypican 3 Protein (Gln 25-His 559) [His]
VAng-1699Lsx-1mg	Creative Biolabs	1 mg	EUR 5998.8
Description: Human Glypican 3 (GPC3), His tag, is expressed in human 293 cells. (Uniprot ID: P51654)

Human Glypican 1 / GPC1 Protein, Fc Tag
GP1-H5254	ACROBIOSYSTEMS	100ug	EUR 2749.9
Description: Human Glypican 1, Fc Tag (GP1-H5254) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # P35052-1).

GPC4 Glypican-4 Human Recombinant Protein
PROTO75487	BosterBio	Regular: 10ug	EUR 380.4
Description: GPC4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids (401-529a.a) and having a molecular mass of 18kDa.GPC4 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

GPC3 Glypican-3 Human Recombinant Protein
PROTP51654	BosterBio	Regular: 10ug	EUR 380.4
Description: GPC3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 544 amino acids (25-559a.a.) and having a molecular mass of 61.8kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). GPC3 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.

Human Glypican 1 / GPC1 Protein, His Tag
GP1-H52H9	ACROBIOSYSTEMS	100ug	EUR 2749.9
Description: Human Glypican 1, His Tag (GP1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # P35052-1).

Human Glypican 2 / GPC2 Protein, His Tag
GP2-H52H3	ACROBIOSYSTEMS	100ug	EUR 2749.9
Description: Human Glypican 2, His Tag (GP2-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gly 554 (Accession # Q8N158-1).

Human glypican 6 (GPC6) ELISA kit
CSB-EL009708HU-24T	Cusabio	1 plate of 24 wells	EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human glypican 6 (GPC6) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human glypican 6 (GPC6) ELISA kit
1-CSB-EL009708HU	Cusabio	EUR 964.80 EUR 6118.80 EUR 3244.80	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human glypican 6 (GPC6) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human glypican 6 (GPC6) ELISA kit
EKC33881-48T	Biomatik Corporation	48T	EUR 535.99

Human glypican 6 (GPC6) ELISA kit
EKC33881-5x96T	Biomatik Corporation	5x96T	EUR 3637.08

Human glypican 6 (GPC6) ELISA kit
EKC33881-96T	Biomatik Corporation	96T	EUR 765.7

Human Glypican 6(GPC6)ELISA Kit
QY-E02274	Qayee Biotechnology	96T	EUR 433.2

Glypican-4, 511 a.a. Protein
20-abx262795	Abbexa	EUR 393.60 EUR 7676.40 EUR 276.00	10 ug 1 mg 2 µg

Human Glypican 3 Protein (Gln 25-His 559) [His] [Avi]
VAng-1701Lsx-200g	Creative Biolabs	200 µg	EUR 4612.8
Description: Biotinylated Human Glypican 3 (GPC3), His tag and Avi tag, is expressed in human 293 cells. (Uniprot ID: P51654-1)

Human Glypican 3 Protein (Gln 25-His 559) [His] [Avi]
VAng-1701Lsx-25g	Creative Biolabs	25 µg	EUR 1215.6
Description: Biotinylated Human Glypican 3 (GPC3), His tag and Avi tag, is expressed in human 293 cells. (Uniprot ID: P51654-1)

Glypican 4 (GPC4) Protein
20-abx262759	Abbexa	EUR 393.60 EUR 7676.40 EUR 276.00	10 ug 1 mg 2 µg

Human Glypican 3 / GPC3 (359-559) Protein, His Tag
GP3-H52H5	ACROBIOSYSTEMS	1mg	EUR 2493.1
Description: Human Glypican 3 (359-559) Protein, His Tag (GP3-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 359 - His 559 (Accession # P51654-1).

Glypican-6 Antibody (HRP)
20-abx108308	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Glypican-6 Antibody (FITC)
20-abx106888	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Glypican-6 Antibody (Biotin)
20-abx105470	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Biotinylated Recombinant Human Glypican-3/GPC3 Protein
RP02371	Abclonal	1000μg	EUR 1423.5

Human Glypican 3 Protein (Gln 25-His 559) [Fc] (FITC-Labeled)
VAng-1698Lsx-200g	Creative Biolabs	200 µg	EUR 4184.4
Description: FITC-Labeled Human Glypican 3 (GPC3), Fc Tag, is expressed in human 293 cells. (Uniprot ID: P51654)

Human Glypican 3 Protein (Gln 25-His 559) [Fc] (FITC-Labeled)
VAng-1698Lsx-25g	Creative Biolabs	25 µg	EUR 1017.6
Description: FITC-Labeled Human Glypican 3 (GPC3), Fc Tag, is expressed in human 293 cells. (Uniprot ID: P51654)

Human Glypican 3 Protein (Gln 25-His 559) [His] (FITC-Labeled)
VAng-1700Lsx-200g	Creative Biolabs	200 µg	EUR 4184.4
Description: FITC-Labeled Human Glypican 3 (GPC3), His tag, is expressed in human 293 cells. (Uniprot ID: P51654-1)

Human Glypican 3 Protein (Gln 25-His 559) [His] (FITC-Labeled)
VAng-1700Lsx-25g	Creative Biolabs	25 µg	EUR 1017.6
Description: FITC-Labeled Human Glypican 3 (GPC3), His tag, is expressed in human 293 cells. (Uniprot ID: P51654-1)

Human Glypican 3 Protein (Gln 25-His 559) [Llama IgG2b Fc Tag]
VAng-1702Lsx-100g	Creative Biolabs	100 µg	EUR 1215.6
Description: Human Glypican 3 (GPC3), Llama IgG2b Fc Tag, is expressed in human 293 cells. (Uniprot ID: P51654-1)

Human Glypican 3 Protein (Gln 25-His 559) [Llama IgG2b Fc Tag]
VAng-1702Lsx-1mg	Creative Biolabs	1 mg	EUR 7682.4
Description: Human Glypican 3 (GPC3), Llama IgG2b Fc Tag, is expressed in human 293 cells. (Uniprot ID: P51654-1)

Rat Glypican 4 (GPC4) Protein
20-abx066895	Abbexa	EUR 910.80 EUR 360.00 EUR 2865.60 EUR 1095.60 EUR 644.40	100 ug 10 ug 1 mg 200 ug 50 ug

Rat Glypican 5 (GPC5) Protein
20-abx650459	Abbexa	EUR 661.20 EUR 292.80 EUR 1914.00 EUR 777.60 EUR 493.20	100 ug 10 ug 1 mg 200 ug 50 ug

Rat Glypican 3 (GPC3) Protein
20-abx653617	Abbexa	EUR 777.60 EUR 326.40 EUR 2397.60 EUR 927.60 EUR 560.40	100 ug 10 ug 1 mg 200 ug 50 ug

Recombinant Human Glypican 1/GPC1 Protein, His Tag
E40KMH1170	EnoGene	20ug	EUR 495

Recombinant Human Glypican 1/GPC1 Protein, His Tag
E40KMH1171	EnoGene	20ug	EUR 495

Recombinant Human Glypican 5/GPC5 Protein, His Tag
E40KMH1172	EnoGene	20ug	EUR 495

Glypican 6 (GPC6) Antibody
20-abx109831	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Glypican 6 (GPC6) Antibody
20-abx212986	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Glypican 6 (GPC6) Antibody
20-abx213390	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Glypican 6 (GPC6) Antibody
20-abx211678	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Glypican 6 (GPC6) Antibody
20-abx005979	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Human Glypican 2 / GPC2 Protein, Fc Tag (SPR verified)
GP2-H5255	ACROBIOSYSTEMS	100ug	EUR 2749.9
Description: Human Glypican 2, Fc Tag (GP2-H5255) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Ser 553 (Accession # Q8N158-1).

Human Glypican 3 / GPC3 (S495A, S509A) Protein, His Tag
GP3-H52H8	ACROBIOSYSTEMS	100ug	EUR 2749.9
Description: Human Glypican 3 (S495A, S509A), His Tag (GP3-H52H8) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1 (S495A, S509A)).

Glypican 1 Recombinant Protein
91-578	ProSci	0.05 mg	EUR 588.3
Description: Glypican-1 is a cell membrane protein and belongs to the glypican family. The protein may act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. It is required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.

Glypican 3 Recombinant Protein
96-360	ProSci	0.1 mg	EUR 682.8
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein
96-803	ProSci	0.1 mg	EUR 852.9
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein
96-804	ProSci	0.1 mg	EUR 651.3
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein
97-001	ProSci	0.1 mg	EUR 682.8
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Glypican 3 Recombinant Protein
97-002	ProSci	0.1 mg	EUR 651.3
Description: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Human Glypican 3 / GPC3 Protein, Fc Tag, low endotoxin
GP3-H5258	ACROBIOSYSTEMS	1mg	EUR 321
Description: Human Glypican 3, Fc Tag, low endotoxin (GP3-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).

Mouse Glypican 3 (GPC3) Protein
20-abx167236	Abbexa	EUR 794.40 EUR 326.40 EUR 2431.20 EUR 944.40 EUR 577.20	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse Glypican 1 (GPC1) Protein
20-abx066890	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse Glypican 1 (GPC1) Protein
20-abx066891	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse Glypican 4 (GPC4) Protein
20-abx066894	Abbexa	EUR 844.80 EUR 343.20 EUR 2631.60 EUR 1011.60 EUR 610.80	100 ug 10 ug 1 mg 200 ug 50 ug

Human Glypican 3 / GPC3 Protein, His Tag, premium grade
GP3-H52H4	ACROBIOSYSTEMS	100ug	EUR 342.4
Description: Human Glypican 3, His Tag (GP3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).

FITC-Labeled Human Glypican 3 / GPC3 Protein, Fc Tag
GP3-HF258	ACROBIOSYSTEMS	25ug	EUR 2471.7
Description: FITC-Labeled Human Glypican 3, Fc Tag (Cat. No. GP3-HF258) is expressed from human HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654-1). It is the FITC labeled form of Human Glypican 3, Fc Tag (Cat. No. GP3-H5258).

Human Glypican 4 / GPC4 Protein, His Tag (MALS verified)
GP4-H52H3	ACROBIOSYSTEMS	100ug	EUR 2814.1
Description: Human Glypican 4, His Tag (GP4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Ser 529 (Accession # O75487-1).

Mouse Glypican-6,GPC-6 ELISA Kit
YLA1311MO-48T	Shanghai YL Biotech	48T	EUR 465

Mouse Glypican-6,GPC-6 ELISA Kit
YLA1311MO-96T	Shanghai YL Biotech	96T	EUR 600

GPC4-511-aa Glypican-4 511 aa Human Recombinant Protein
PROTO75487-1	BosterBio	Regular: 10ug	EUR 380.4
Description: Glypican-4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Ala19-Ser529) containing 521 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 58.7kDa.

FITC-Labeled Human Glypican 3 / GPC3 Protein, His Tag
GP3-HF2H1	ACROBIOSYSTEMS	25ug	EUR 342.4
Description: FITC-Labeled Human Glypican 3, His Tag (Cat. No. GP3-HF2H1) is expressed from human HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654-1). It is the FITC labeled form of Human Glypican 3, His Tag (Cat. No. GP3-H52H4).

Cynomolgus Glypican 3 Protein (Gln 25-His 559) [human IgG1 Fc tag]
VAng-1652Lsx-100g	Creative Biolabs	100 µg	EUR 1017.6
Description: Cynomolgus Glypican 3 (GPC3), human IgG1 Fc tag, is expressed in human 293 cells. (Uniprot ID: XP_005594665)

Cynomolgus Glypican 3 Protein (Gln 25-His 559) [human IgG1 Fc tag]
VAng-1652Lsx-1mg	Creative Biolabs	1 mg	EUR 5998.8
Description: Cynomolgus Glypican 3 (GPC3), human IgG1 Fc tag, is expressed in human 293 cells. (Uniprot ID: XP_005594665)

Human Glypican-1 Recombinant Protein N-6His Tag Lyophilized
IHUGPC1RN6HISLY10UG	Innovative research	each	EUR 133
Description: Human Glypican-1 Recombinant Protein N-6His Tag Lyophilized

Glypican 2 / GPC2 Recombinant Protein
11-254	ProSci	0.1 mg	EUR 714.3
Description: Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.

Glypican 1 / GPC1 Recombinant Protein
11-321	ProSci	0.1 mg	EUR 714.3
Description: Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Glypican 2 / GPC2 Recombinant Protein
11-322	ProSci	0.1 mg	EUR 714.3
Description: Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.

Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin-derived peptide

Polyvinylidene fluoride (PVDF) is biocompatible, easy to fabricate, and has piezoelectric properties; it has been used for many biomedical applications including stem cell engineering. However, long-term cultivation of human embryonic stem cells (hESCs) and their differentiation toward cardiac lineages on PVDF have not been investigated.

Herein, PVDF nanoscaled membrane scaffolds were fabricated by electrospinning; a vitronectin-derived peptide-mussel adhesive protein fusion (VNm) was immobilized on the scaffolds. hESCs cultured on the VNm-coated PVDF scaffold (VNm-PVDF scaffold) were stably expanded for more than 10 passages while maintaining the expression of pluripotency markers and genomic integrity.

Under cardiac differentiation conditions, hESCs on the VNm-PVDF scaffold generated more spontaneously beating colonies and showed the upregulation of cardiac-related genes, compared with those cultured on Matrigel and VNm alone. Thus, VNm-PVDF scaffolds may be suitable for the long-term culture of hESCs and their differentiation into cardiac cells, thus expanding their application in regenerative medicine.